15个鸡群体IL-2基因的遗传变异分析

doi:10.11843/j.issn.0366-6964.2015.01.006

摘要/Abstract

摘要：

为了深入研究鸡IL-2基因的多态性及为鸡抗病育种提供理论依据，本研究以IL-2基因为研究对象，采用直接测序的方法对11个中国地方鸡品种、3个引进鸡品种和1个培育鸡品种共448个个体该基因的遗传变异进行了分析。通过DNA池和直接测序技术，共发现了13个突变位点。对所有个体的一段包含5个SNPs的区域进行分析，发现不同等位基因在15个鸡群体间分布存在显著差异，表明不同品种间IL-2基因具有丰富的遗传变异资源，可为抗病基因的选择提供素材；群体遗传学指标分析表明，H系京星黄鸡的遗传多样性最低，这与其品种形成过程一致，其他品种多样性差异不大；单倍型分析共发现23种单倍型，其中H1(CAACG)、H2(CAATT)、H4(TGTTG)、H9(TGTTG)、H11(TATCG)和H16(CATCG)为中国地方鸡品种特有或优势单倍型，可能与抗病能力相关。

Abstract:

To deeply study the genetic diversity of chicken Interleukin 2 (IL-2) gene and provide a theoretic basis for the breeding for disease resistance，the genetic variation of IL-2 were studied in 448 individuals from 11 Chinese native，3 introduced and one cultivated chicken breeds by DNA sequencing method in this study.A total of 13 SNPs were identified by using DNA-pooling and sequencing.The results of analysis for a genome region including 5 single nucleotide polymorphisms(SNPs) among all the individuals showed that the frequencies of different alleles differed significantly among 15 populations，suggesting IL-2 had abundant genetic variations and provided promising gene resource for disease resistance.Analysis of several population genetic indexes indicated that the Jingxing Yellow chicken had the lowest level of genetic diversity,which was consistent with its breed formation and little differences of genetic diversity were observed among other breeds.Analysis of haplotypes revealed a total of 23 hapltoyepes.Among them，H1(CAACG)，H2(CAATT)，H4(TGTTG)，H9(TGTTG)，H11(TATCG) and H16(CATCG) were unique or dominant in Chinese native chicken breeds，suggesting these haplotypes may be associated with the disease-resistant ability.

中图分类号:

S831.2

赵会静，张敬敬，文杰，刘冉冉，郑麦青，李庆贺，马月辉，赵桂苹. 15个鸡群体IL-2基因的遗传变异分析[J]. 畜牧兽医学报, 2015, 46(1): 41-49.

ZHAO Hui-jing，ZHANG Jing-jing,WEN Jie,LIU Ran-ran,ZHENG Mai-qing,LI Qing-he,MA Yue-hui,ZHAO Gui-ping. Genetic Diversity of IL-2 Gene in 15 Chicken Populations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 41-49.

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